SLICElactase+lab

Lactase Lab Report

Name:

Explain in words what occurs in a lactase catalyzed reaction. There is something called lactose that is glactose + glucose. This cannot be digested because it is to big. The lactase breaks it down so it can be absorbed.

Explain in words what occurs in a when a person is lactose intolerant. They cannot absorb all the lactose and it just stays in the intestines so the body sends in water to dilute it and you have moist stool

Basic Instructions: •	Wear safety glasses. •	Turn on hot plate. •	Add contents using separate pipet for each ingredient. •	Make tubes with enzyme first so the enzyme has time to work. •	Label tubes with number. •	After adding ingredients, mix with new piece of parafilm on top.

Tube	Contents	Purpose	Result	Conclusion 1	Milk 3ml Enzyme 2 ml Benedicts Reagent 10 Drops	Expect orange color because the enzyme should digest lactose	Glucose was produced	Strip turned green 2	Water 3 ml Enzyme 2ml Benedicts Reagent 10 Drops	Stay blue because there is no lactose to break down	No glucose to break down	Stayed orange 3	Milk 3ml Water 2ml Benedicts Reagent 10 Drops	Stay blue because there is no enzyme to break down the lactose	No glucose	Stayed orange 4	Sucrose 3ml Enzyme 2ml Benedicts Reagent 10 Drops	Stay blue because lactase cannot digest sucrose	Lactase cannot break down sucrose	Stayed orange 5	Glucose 5 ml Benedicts Reagent 10 Drops	Should turn orange, positive control	Glucose control	we were right green